@article{Carleo_Landi_Prasse_Bergantini_d’Alessandro_Cameli_Janciauskiene_Rottoli_Bini_Bargagli_2020, title={Proteomic characterization of idiopathic pulmonary fibrosis patients: stable versus acute exacerbation}, volume={90}, url={https://www.monaldi-archives.org/macd/article/view/1231}, DOI={10.4081/monaldi.2020.1231}, abstractNote={<p>Acute exacerbations (AEs) are among the main causes of death in idiopathic pulmonary fibrosis (IPF) patients. In this study proteomic comparative analysis of bronchoalveolar lavage (BAL) fluid samples was performed in stable IPF patients <em>versus</em> AEs IPF group to identify AE pathogenetic mechanisms and novel potential predictive biomarkers. A functional proteomic analysis of BAL fluid samples from stable and AE-IPF patients was conducted in a population of 27 IPF patients. Fifty-one differentially abundant spots were observed and identified by mass spectrometry. Enrichment analysis found proteins of interest involved in the regulation of macrophages and lipid metabolism receptors. In acute exacerbation IPF group, differentially abundant proteins were involved in propagation of the β-catenin WNT transduction signal, and proteins up-regulated in lung carcinogenesis (IGKC, S100A9, PEDF, IGHG1, ALDOA, A1AT, HPT, CO3 and PIGR) and acute phase proteins involved in protease-antiprotease imbalance (such as A1AT fragments). Dot-blot analysis of A1AT C-36 peptide allowed validating our findings, confirming up-regulation in AE IPF patients and suggesting its potential pathogenetic role. A crucial role of protease/antiprotease imbalance, clathrin-mediated endocytosis signalling and carcinogenesis emerged in IPF patients developing acute exacerbations.</p>}, number={2}, journal={Monaldi Archives for Chest Disease}, author={Carleo, Alfonso and Landi, Claudia and Prasse, Antje and Bergantini, Laura and d’Alessandro, Miriana and Cameli, Paolo and Janciauskiene, Sabina and Rottoli, Paola and Bini, Luca and Bargagli, Elena}, year={2020}, month={Apr.} }